The most widely used detection for pesticides in grapes is combined with gas or liquid
chromatography. The cultivation of grape is spread widely in the world. The production of grapes is
important as it contains various nutritional properties. it is consumed in fresh form as well as in
processed products such as jam, jelly, wine, beer, raisins, vinegar and grape seed oil.
With the production of grapes, pesticides are used for the protection and controlling of pests and
diseases. The most common fungal disease are powdery mildew, downy mildew and gray mold and
insects found are European grapevine moth, vine mealy bug and the citrus mealy bug .
Use Of Chromatography In Detection
In 2008, gas chromatography couple to mass spectrometry and liquid chromatography to tandem mass
spectrometry were published, where grapes were tested as one of the representative’s fruit matrices.
The Association of Official Analytical Chemist also published a method for detection of pesticides
with a simple model treatment and later treated by gas chromatography and liquid chromatography
The grapes was also used in detection pesticides in fruit and vegetables with a common extraction
steps and clean up followed by chromatography.
Improvements In Analysis Of Pesticides
In last 10 years, many improvements have been made for analysis of pesticides in grapes. Some
Reduction in sample size and quantity of organic solvents.
The automation of sample preparation which helped in reductions of errors in the
manipulation and helped in reproducibility.
Better separation techniques leading to an increased resolution and reduced separation time.
The QuEChERS methodology is the most common technique. The instrument of choice for analytical
separation are both liquid chromatography and gas chromatography. These chromatography are
coupled to a tandem MS/MS as QqQ for identification and quantification.